@article {10.3844/ajavsp.2023.304.316,
article_type = {journal},
title = {Effect of Temperature Change and Estradiol Levels on Cattle Oocytes Developmental Competency: Comparing X-Sexed and Non-Sexed Sperm Following in vitro Fertilization},
author = {Sebopela, Maleke Dimpho and Mphaphathi, Masindi Lottus and Sithole, Sindisiwe Mbali and Chokoe, Tlou Caswell and Nedambale, Tshimangadzo Lucky},
volume = {18},
number = {4},
year = {2023},
month = {Nov},
pages = {304-316},
doi = {10.3844/ajavsp.2023.304.316},
url = {https://thescipub.com/abstract/ajavsp.2023.304.316},
abstract = {The study aimed to investigate the effects of temperature change and estradiol (E2) hormone level on In Vitro Maturation (IVM) and fertilization of cattle oocytes using X-sexed and non-sexed sperm. In experiment 1: The oocytes were incubated at various temperatures of 35.5, 36.5, 37.5, 38.5, 39.5 and 40.5°C under similar conditions with 5% CO2, in humidified air for 22 h. In experiment 2: The IVM medium was supplemented with various concentrations of E2 (0, 0.1, 0.2, 0.3, 0.4 and 0.5 µg/mL). In experiment 3: The matured oocytes were fertilized in vitro using X-sexed and non-sexed frozen-thawed semen. An Analysis of Variance (ANOVA) was performed. Comparisons were considered significantly different at (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}