@article {10.3844/ajavsp.2023.27.39, article_type = {journal}, title = {Comparison of in vitro Maturation Media on Cattle Oocytes after in vitro Embryo Production}, author = {Sithole, Sindisiwe Mbali and Mphaphathi, Masindi Lottus and Sebopela, Maleke Dimpho and Nedambale, Tshimangadzo Lucky}, volume = {18}, number = {1}, year = {2023}, month = {Feb}, pages = {27-39}, doi = {10.3844/ajavsp.2023.27.39}, url = {https://thescipub.com/abstract/ajavsp.2023.27.39}, abstract = {The study was conducted to compare differences in vitro maturation media and their effect on subsequent in vitro embryo production. The study procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) and the Tshwane university of technology animal research ethics committee (Ref no: AREC 2021/08/004). Follicular fluid containing a suspension of oocytes was retrieved from ovaries through aspiration and slicing techniques. The retrieved oocytes were graded and randomly matured into four media (TCM199, Vitromat Protect, and BO IVM®) groups for 22 h, evaluated for polar body extrusion, fertilized through their respective in vitro fertilization media, and evaluated for pronucleus status. Presumptive zygotes were further cultured in vitro in their respective media, incubated for either 18 or 96 h, and evaluated for cleavage rates. The data was subjected to an appropriate analysis of variance. Student's Least Significant Differences (t-LSD) was calculated at a 5% significance level to compare means of significant treatment effects. The BO-IVM® (57.4%), TCM 199 (55.6%), and VitroMat Protect® (54.0%) recorded higher results than EGF (26.9%) for polar body formation on oocytes (P}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }