@article {10.3844/ajavsp.2023.261.272, article_type = {journal}, title = {In vitro Ruminal Cumulative Gas and Methane Production, Enzyme Activity, Fermentation Profile and Nutrient Digestibility on Feed Supplemented with Organic Selenium}, author = {Sofiā€™ul Anam, Moh. and Astuti, Andriyani and Widyobroto, Budi Prasetyo and Gunawan, and Agus, Ali}, volume = {18}, number = {4}, year = {2023}, month = {Oct}, pages = {261-272}, doi = {10.3844/ajavsp.2023.261.272}, url = {https://thescipub.com/abstract/ajavsp.2023.261.272}, abstract = {Methane (CH4) emissions are indeed a global environmental concern and ruminants are a significant source of these emissions; however, there is limited understanding regarding the contribution of organic Selenium (Se) in mitigating CH4 emissions. Hence, this study investigated the impact of supplementing organic Se from Selenomethionine (Se-Met) on the in vitro ruminal cumulative gas and CH4 production, fermentation profile, enzyme activities, and nutrient digestibility. The trial protocol has been approved by the ethical clearance committee and encompassed four distinct treatments implemented within a completely randomized design. The treatments were described in the following manner: (1) Basic feed without additional substances (CTL); (2) CTL with 0.15 mg of Se per kg of Dry Matter (DM)-denoted as low Se-Met (LS); (3) CTL with 0.30 mg of Se per kg DM-referred to as medium Se-Met (MS); and (4) CTL with 0.45 mg of se per kg DM- termed high Se-Met (HS). Each group consisted of five replications and was randomly allocated to the in vitro tubes. The research was conducted comprehensively for two months. The data underwent analysis using the General Linear Model (GLM) procedure and a probability value of p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }