TY  - JOUR
AU  - Martins, Nathálya dos Santos 
AU  - Sousa Rodrigues, Ana Paula 
AU  - Gonçalves, Stefanne Aparecida 
AU  - Abreu Silva, Ana Lucia 
AU  - de Oliveira, Rudson Almeida 
AU  - dos Reis, Jenner Karlisson Pimenta 
AU  - Melo, Ferdinan Almeida 
PY  - 2015
TI  - Occurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MA
JF  - American Journal of Animal and Veterinary Sciences
VL  - 10
IS  - 3
DO  - 10.3844/ajavsp.2015.187.192
UR  - https://thescipub.com/abstract/ajavsp.2015.187.192
AB  - The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. To detect anti-FIV antibodies and the p27antigen of FeLV, the samples were tested with a commercial immunoassay kit, SNAP Combo Plus (IDEXX® Laboratories, USA), an indirect ELISA was also carried out for FIV. All three tests used gave 18.33% (22/120) of positivity for FIV. PCR and indirect ELISA found 10.83% (13/120) of the animals with FIV, while the SNAP Combo Plus found 9.17% (11/120). Only 0.83% (1/120) of the samples were positive for FeLV in the SNAP combo plus. Comparatively, SNAP combo plus showed higher sensitivity, whereas the indirect ELISA and PCR had, similar and better specificity (p