TY  - JOUR
AU  - Gonzalez-Marin, Clara 
AU  - Kjelland, Michael Eduard 
AU  - Roy, Rosa 
AU  - Lopez-Fernandez, Carmen 
AU  - Fernandez, Jose Luis 
AU  - Moreno, Juan Fernando 
AU  - Gosalvez, Jaime 
PY  - 2012
TI  - Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage
JF  - American Journal of Animal and Veterinary Sciences
VL  - 7
IS  - 4
DO  - 10.3844/ajavsp.2012.180.185
UR  - https://thescipub.com/abstract/ajavsp.2012.180.185
AB  - Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based on the presence or absence of bacteria: One group (n = 10) had a readily detectable bacterial presence, while the other group (n = 10) had an undetectable bacterial presence. Differences in the SDF at T0 were not observed between these two groups. However, statistically different results were found after 24 h of incubation at 37°C (Kaplan-Meier estimator; Log-Rank Matel-Cox, p0.05) were not detected between the control and the quinolone treated sample for those samples without bacteria. However, differences (p