@article {10.3844/ajavsp.2012.180.185, article_type = {journal}, title = {Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage}, author = {Gonzalez-Marin, Clara and Kjelland, Michael Eduard and Roy, Rosa and Lopez-Fernandez, Carmen and Fernandez, Jose Luis and Moreno, Juan Fernando and Gosalvez, Jaime}, volume = {7}, number = {4}, year = {2012}, month = {Dec}, pages = {180-185}, doi = {10.3844/ajavsp.2012.180.185}, url = {https://thescipub.com/abstract/ajavsp.2012.180.185}, abstract = {Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based on the presence or absence of bacteria: One group (n = 10) had a readily detectable bacterial presence, while the other group (n = 10) had an undetectable bacterial presence. Differences in the SDF at T0 were not observed between these two groups. However, statistically different results were found after 24 h of incubation at 37°C (Kaplan-Meier estimator; Log-Rank Matel-Cox, p0.05) were not detected between the control and the quinolone treated sample for those samples without bacteria. However, differences (p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }