TY  - JOUR
AU  - Santos, Elizangela Maira Dos 
AU  - Motta, Pedro Moreira Couto 
AU  - Heinemann, Marcos Bryan 
AU  - Leite, Rômulo Cerqueira 
AU  - Puerto, Helen Lima Del 
AU  - Reis, Jenner Karlisson Pimenta Dos 
PY  - 2010
TI  - EIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar Wash
JF  - American Journal of Animal and Veterinary Sciences
VL  - 5
IS  - 3
DO  - 10.3844/ajavsp.2010.180.182
UR  - https://thescipub.com/abstract/ajavsp.2010.180.182
AB  - Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectious
disease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experiment
was compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) and
Bronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samples
isolated from PBMCs and BAW of naturally infected animals were used for a nested PCR amplifying a
408 bp gag gene fragment region. Equine Dermal cells (ED) infected and non infected by EIAV was
used as nested PCR negative and positive control. Horses’ blood samples were firstly tested as positive in Agar Gel Immunodiffusion Assay (AGID). Results: Results demonstrated 89% (51/57) of gag gene amplification in PBMCs samples and only 47% (27/57) of gag gene amplification in BAW samples. Conclusion: The nested PCR assay used in the present study detected more EIAV positive samples in PBMC than in BAW, indicating PBMCs as a reliable source for EIAV diagnosis.