@article {10.3844/ajavsp.2010.180.182,
article_type = {journal},
title = {EIAV Effectiveness Detection by Nested PCR Comparing two Different Samples: PBMCs and Bronchoalveolar Wash},
author = {Santos, Elizangela Maira Dos and Motta, Pedro Moreira Couto and Heinemann, Marcos Bryan and Leite, Rômulo Cerqueira and Puerto, Helen Lima Del and Reis, Jenner Karlisson Pimenta Dos},
volume = {5},
number = {3},
year = {2010},
month = {Sep},
pages = {180-182},
doi = {10.3844/ajavsp.2010.180.182},
url = {https://thescipub.com/abstract/ajavsp.2010.180.182},
abstract = {Problem statement: Equine Infectious Anemia (EIA) is a chronic, relapsing infectious
disease of horses caused by Equine Infectious Anemia Virus (EIAV). The objective of this experiment
was compared EIAV effectiveness detection in Peripheral Blood Mononuclear Cells (PBMCs) and
Bronchoalveolar wash (BAW) from naturally infected horses. Approach: Fifty seven DNA samples
isolated from PBMCs and BAW of naturally infected animals were used for a nested PCR amplifying a
408 bp gag gene fragment region. Equine Dermal cells (ED) infected and non infected by EIAV was
used as nested PCR negative and positive control. Horses’ blood samples were firstly tested as positive in Agar Gel Immunodiffusion Assay (AGID). Results: Results demonstrated 89% (51/57) of gag gene amplification in PBMCs samples and only 47% (27/57) of gag gene amplification in BAW samples. Conclusion: The nested PCR assay used in the present study detected more EIAV positive samples in PBMC than in BAW, indicating PBMCs as a reliable source for EIAV diagnosis.},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}