TY  - JOUR
AU  - Ghazanfari, Shokoufe 
AU  - Nassiry, Mohammad Reza 
AU  - Tahmoorespur, Mojtaba 
AU  - Salehi, Abdolreza 
AU  - Nobari, Karim 
PY  - 2010
TI  - Ghrelin Gene Expression in Broiler Proventriculus Tissue are Changed by Feed Restriction, Different Dietary Energy and Protein Levels
JF  - American Journal of Animal and Veterinary Sciences
VL  - 5
IS  - 3
DO  - 10.3844/ajavsp.2010.175.179
UR  - https://thescipub.com/abstract/ajavsp.2010.175.179
AB  - Problem statement: The aim of this study was to investigate the effects of feed restriction
and different energy and protein contents of diet on ghrelin gene expression in broiler chicken.
Approach: Feeding programs consisted of ad libitum and feed restriction, two energy levels (3100 and
2800 kcal ME kg-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction was applied
during 22-32 days of age. Proventriculus samples were collected at 21, 32 and 49 days of age. Ghrelin
mRNA expression in proventriculus tissue was quantitate using Real Time quantitative PCR. Results: We
found that ghrelin gene expression was increased in restricted chicks compared with those fed ad
libitum at 32 days of age (p = 0.09) but feed restriction had no effect on ghrelin gene expression at 49
days of age. A positive response in ghrelin gene expression was achieved by decreasing energy level in
the diet at 21 days of age (p