@article {10.3844/ajavsp.2010.175.179,
article_type = {journal},
title = {Ghrelin Gene Expression in Broiler Proventriculus Tissue are Changed by Feed Restriction, Different Dietary Energy and Protein Levels},
author = {Ghazanfari, Shokoufe and Nassiry, Mohammad Reza and Tahmoorespur, Mojtaba and Salehi, Abdolreza and Nobari, Karim},
volume = {5},
number = {3},
year = {2010},
month = {Sep},
pages = {175-179},
doi = {10.3844/ajavsp.2010.175.179},
url = {https://thescipub.com/abstract/ajavsp.2010.175.179},
abstract = {Problem statement: The aim of this study was to investigate the effects of feed restriction
and different energy and protein contents of diet on ghrelin gene expression in broiler chicken.
Approach: Feeding programs consisted of ad libitum and feed restriction, two energy levels (3100 and
2800 kcal ME kg-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction was applied
during 22-32 days of age. Proventriculus samples were collected at 21, 32 and 49 days of age. Ghrelin
mRNA expression in proventriculus tissue was quantitate using Real Time quantitative PCR. Results: We
found that ghrelin gene expression was increased in restricted chicks compared with those fed ad
libitum at 32 days of age (p = 0.09) but feed restriction had no effect on ghrelin gene expression at 49
days of age. A positive response in ghrelin gene expression was achieved by decreasing energy level in
the diet at 21 days of age (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}