@article {10.3844/ajabssp.2010.468.476,
article_type = {journal},
title = {Use of a Mixture of Thermophilic Enzymes Produced by the Fungus Thermoascus aurantiacus to Enhance the Enzymatic Hydrolysis of the Sugarcane Bagasse Cellulose},
author = {Monte, J. R. and Carvalho, W. and Milagres, A. M. F.},
volume = {5},
year = {2010},
month = {Dec},
pages = {468-476},
doi = {10.3844/ajabssp.2010.468.476},
url = {https://thescipub.com/abstract/ajabssp.2010.468.476},
abstract = {Problem statement: The production of hydrolytic enzymes by T. aurantiacus has been
performed under solid-state fermentations using lignocellulosic materials. The influences of the
inoculum size and of the fermentation medium on the production of hemicellulases and cellulases were
studied. Filtrates from the cultures were used to hydrolyze a pulp of sugarcane bagasse and the
produced enzymes were shown to be candidates for use as co-adjuvants in plant saccharification.
Aproach: The present study focuses on the effect of different culture conditions on production of
cellulases and hemicellulases by T. aurantiacus. It is also provides a possible application of
T. aurantiacus enzymes in the degradation of sugarcane bagasse pulp, considering that this
thermophilic fungus is a potential source of thermostable enzymes. Results: T. aurantiacus was
cultivated on four different agricultural residues: sugarcane bagasse, sugarcane straw, wheat straw and
corn cob. Xylanase was produced with much more expressive activity than cellulases. The highest titre
of xylanase was obtained on sugarcane straw at 9 days (1679.8 IU g−1); the same was observed for β-
glucosidase (29.9 IU g−1) at 6 days. With an inoculum load of 108 spores g−1, the amount of
exoglucanase produced by the fungus considerably exceeds that produced with 104 spores g−1.
Xylanases and cellulases purified from filtrates of the cultures were investigated to hydrolyze a
bagasse pulp prepared with alkaline peroxide. Xylanase or sulphuric acid were used as pretreatments
for xylan removal, increasing the cellulase performance on pulp bagasse. However, results revealed
that the removal of hemicellulose is not the only main factor limiting the cellulose hydrolysis.
Conclusion: Results indicate that the xylanase action on alkaline-pretreated sugar cane bagasse
enhances the cellulolytic effect promoted by a commercial cellulase. This study thus presents an
evaluation of the applicability of enzymes from Thermoascus aurantiacus to potentially improve the
enzymatic cellulose hydrolysis.},
journal = {American Journal of Agricultural and Biological Sciences},
publisher = {Science Publications}
}