@article {10.3844/ajabssp.2009.72.78,
article_type = {journal},
title = {Biocontrol of Botrytis allii Munn the Causal Agent of Neck Rot, the Post Harvest Disease in Onion, by use of a New Iranian Isolate of Streptomyces},
author = {Jorjandi, M. and Bonjar, G.H. Shahidi and Baghizadeh, A. and Sirchi, G.R. Sharifi and Massumi, H. and Baniasadi, F. and Aghighi, S. and Farokhi, P. Rashid},
volume = {4},
year = {2009},
month = {Mar},
pages = {72-78},
doi = {10.3844/ajabssp.2009.72.78},
url = {https://thescipub.com/abstract/ajabssp.2009.72.78},
abstract = {Problem statement: Soil actinomycetes particularly Streptomyces 
spp. have showed antagonistic activity against wide range of plant pathogens. In the 
recent decades they have attracted high interests as biocontrol agents. Onion 
neck rot or gray mold caused by Botrytis allii have imposed economic post harvest 
damages to onion bulbs and decreased its storage durability and market value. 
Approach: To investigate for biocontrol means against the pathogen, 
antagonistic activity of 50 isolates of soil Actinomycetes were assayed through 
agar disk method and dual culture bioassays. Active isolates were exposed to chloroform 
for detection of antibiotic. Minimum Inhibitory Concentration (MIC) value and 
solubility of active crude extract in organic solvents were determined for Streptomyces 
isolate No. 347 which showed a unique and stable property of inhibiting Botrytis 
allii. To investigate the antagonistic effect of Streptomyces isolate No 347 
on control of onion gray mold, four different treatments were tested by means of 
Tukey HSD test. Results: From the tested isolates, 13 showed 
anti gray mold activities. Exposure of active isolates to chloroform revealed 
that Streptomyces isolates No 347, 263 and 350 retained their antifungal activities. 
The active metabolite(s) of Streptomyces isolate No 347 was polar, soluble in 
H2O but insoluble in chloroform and methanol. MIC of the crude was determined 
as 0.05 mg mL-1 against B. allii. Stability of the active crude in distilled water 
at room temperature (12-30°C) was about 6 months. Statistical studies indicated 
that Streptomyces isolates No 347 can decrease losses of neck rot with significant 
level (p},
journal = {American Journal of Agricultural and Biological Sciences},
publisher = {Science Publications}
}