TY  - JOUR
AU  - Howard, Z.R. 
AU  - Moore, R.W. 
AU  - Zabala Diaz, I.B. 
AU  - Kim, W.K. 
AU  - Birkhold, S.G. 
AU  - Byrd, J.A. 
AU  - Kubena, L.F. 
AU  - Nisbet, D.J. 
AU  - Ricke, S.C. 
PY  - 2007
TI  - Inoculation of a Poultry Isolate Salmonella enteritidis on Egg Vitelline Membrane: Survival and Growth in Egg Components after Different Refrigeration Storage Times
JF  - American Journal of Agricultural and Biological Sciences
VL  - 2
IS  - 2
DO  - 10.3844/ajabssp.2007.123.129
UR  - https://thescipub.com/abstract/ajabssp.2007.123.129
AB  - An in vitro study was designed to determine the extent of Salmonella enterica serovar enteritidis survival and growth permissiveness in egg components isolated from shell eggs held at refrigeration temperature over an 8 week time period. Eggs were collected from a commercial laying facility at one-week intervals for eight weeks and stored at refrigeration temperature. After storage, eggs were dipped in ethanol, cracked aseptically and separated into yolk and albumen samples. S. enteritidis resistant to novobiocin and nalidixic acid were inoculated on to the surface of the yolk membrane at a concentration of approximately 106 CFU mL-1. Yolks were then covered with albumen and incubated for 24 hrs at 25°C. After incubation, eggs were separated into component parts. Samples were removed from yolk, albumen and yolk membrane and diluted 10-fold in sterile phosphate buffered saline. In albumen, S. enteritidis counts were increased in weeks 3 and 8 compared to week 1 (trial 2). The frequency of eggs exhibiting net growth of S. enteritidis in albumen occurred at week 7 versus weeks 0 and 1 in trial 1 and weeks 3 and 8 versus weeks 0 and 2 in trial 2. In the membrane fraction, the frequency of eggs exhibiting net growth of S. enteritidis occurred at weeks 5 and 8 versus week 0 in trial 2. In the yolk fractions, S. enteritidis counts recovered from week 6 eggs were significantly higher (P