Research Article Open Access

Magnum Opus: Clustered Regularly Interspaced Short Palindromic Repeats Biology and Prokaryotic Gene Silencing

Prem Saran Tirumalai1 and Ashim Kumar Bagchi2
  • 1 Uttam Institute of Technology and Management, India
  • 2 University of Manitoba, Canada

Abstract

Gene Silencing was a technology that was established in eukaryotic system a decade ago and is being used as a research tool widely. However, prokaryotic gene silencing was not workable, till recently a team of researchers from the University of Georgia have proved it possible. Where they have shown that short motif sequences determines the targets of the prokaryotic Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) defence system is regulated by RNA guided Cas protein complex. Thus role of CRISPR system in microbial defense against foreign genetic material (Plasmid or Phages) is an important milestone in the field of microbial molecular biology/biotechnology. These findings will make it easier to understand the significance of a gene, metabolically or physiologically. The revelation by this novel finding by core group of researcher is indeed, Mangum opus. This article is a commentary, to bring to light, prokaryotic gene silencing as one of the latest advances in prokaryotic science.

American Journal of Immunology
Volume 9 No. 4, 2013, 110-115

DOI: https://doi.org/10.3844/ajisp.2013.110.115

Submitted On: 24 September 2013 Published On: 5 October 2013

How to Cite: Tirumalai, P. S. & Bagchi, A. K. (2013). Magnum Opus: Clustered Regularly Interspaced Short Palindromic Repeats Biology and Prokaryotic Gene Silencing. American Journal of Immunology, 9(4), 110-115. https://doi.org/10.3844/ajisp.2013.110.115

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Keywords

  • Gene Silencing
  • Prokaryotes
  • CRISPR
  • RNAi
  • Microbial Immunity