Research Article Open Access

Adsorption Behavior of Bone Morphogenetic Protein-2 on a Graphite Substrate for Biomedical Applications

Izabele Marquetti1 and Salil Desai1
  • 1 North Carolina A&T State University, United States
American Journal of Engineering and Applied Sciences
Volume 11 No. 2, 2018, 1037-1044

DOI: https://doi.org/10.3844/ajeassp.2018.1037.1044

Submitted On: 8 May 2018 Published On: 19 June 2018

How to Cite: Marquetti, I. & Desai, S. (2018). Adsorption Behavior of Bone Morphogenetic Protein-2 on a Graphite Substrate for Biomedical Applications. American Journal of Engineering and Applied Sciences, 11(2), 1037-1044. https://doi.org/10.3844/ajeassp.2018.1037.1044

Abstract

The interaction of proteins with biomaterials plays an important role for several biomedical applications including implants and tissue engineering. Although different experimental approaches have been explored, the investigation of the molecular models for protein-substrate interaction is warranted to control of cellular functions. In this study, Molecular Dynamics (MD) simulations were used to study the adsorption behavior of Bone Morphogenetic Protein-2 (BMP-2) on a hydrophobic graphite substrate. The influence of four different orientations of the protein with the substrate was evaluated based on their adsorption behavior. Results indicate a strong influence of the initial configuration on the adsorption. Protein unfolding was observed for most orientations with preferential binding of residues within the vicinity of the substrate. Most of the secondary structure of the protein was preserved during the simulation with a minor reduction of β-sheet structures. This research provides a detailed understanding of the interaction of BMP-2 with biomaterial surfaces.

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Keywords

  • Biomaterials
  • Biomedical Implants
  • Bone Morphogenetic Protein-2
  • Graphite
  • Molecular Dynamics
  • Protein Adsorption