Technique for In-Vivo Measurements of Heart Deformation Using Digital Image Correlation
- 1 Union College, United States
- 2 Research and Advanced Development Group, United States
- 3 McGill University, Canada
Copyright: © 2020 Ronald B. Bucinell, Matthew T. Adams, Mcolisi Dlamini and Leo J. Fleishman. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Many techniques used to measure the deformation of biological tissue are inadequate for measuring deformations of hearts in vivo. Bull Frogs (Rana Catesbeiana) were double-pithed and dissected to expose the amphibian heart for the measurements conducted in this study. White titanium dioxide powder and black charcoal were applied to the surface of the heart to create an artificial surface pattern with high contrast that does not react with the heart muscle or mask surface details. This pattern was then used in conjunction with three dimensional Digital Image Correlation (DIC) to measure full field deformations of the heart through several cardiac cycles. These deformations were measured with a spatial resolution of approximately 0.4 µm and temporal resolution of 50 Hz.
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- 3D DIC
- Heart Deformation
- In vivo Measurements
- Speckle Pattern