Research Article Open Access

Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria

Akmoussi-Toumi Siham1, Khemili-Talbi Souad1, Kebbouche-Gana Salima1, Lenchi-Izouine Nesrine2, Khelfaoui Mohamed Amine1, Sayah Amna1, Bouarab Ghania1, Ferrioune Imen2, Mokhtari Wafa1 and Najjari Afef3
  • 1 Université M’Hamed Bougara, Algeria
  • 2 Université Benyoucef Benkheda, Algeria
  • 3 Université de Tunis El Manar, Tunisia

Abstract

At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these ecosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced hydrolases at high salt concentrations. The Production yields obtained are very promising for applications in the biotechnology and industrial microbiology.

Current Research in Bioinformatics
Volume 8 No. 1, 2019, 46-54

DOI: https://doi.org/10.3844/ajbsp.2019.46.54

Submitted On: 25 December 2019 Published On: 28 February 2020

How to Cite: Siham, A., Souad, K., Salima, K., Nesrine, L., Amine, K. M., Amna, S., Ghania, B., Imen, F., Wafa, M. & Afef, N. (2019). Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria. Current Research in Bioinformatics, 8(1), 46-54. https://doi.org/10.3844/ajbsp.2019.46.54

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Keywords

  • Chott
  • Halophiles
  • 16S rRNA
  • Phylogenic Analysis
  • MEGA6.06