Research Article Open Access

Seedling Propagation of Kulo Chrysanthemum by Tissue Culture

Jeany Polii Mandang1
  • 1 Departement of Agriculture, Faculty of Agriculture, Sam Ratulangi University, Indonesia

Abstract

Aims of this research were to get the right dosage of Plant Growth Regulkator (PGR) and Coconut Water (CW) for shoot multiplication of Kulo chrysanthemums. The study was conducted using a factorial design of three factors namely IAA (0.0; 0.5 and 1.0 ppm), BAP (0, 0.5 and 1.0 ppm) and coconut water (0.0 and 30%). The explants used were clean micro cuttings and were cultured on Murashige and Skoog (MS) media. The media was added IAA, BAP and coconut water. The result showed that the best shoot multiplication of Kulo chrysanthemums was the MS media using combination of 0.5 ppm and 1.0 of IAA, 0.5 ppm of BAP and 30% of coconut water. Good rooting was revealed by the combination of IAA 0.5 and 1 ppm with 30% coconut water and BAP 1 ppm and 30% coconut water.

American Journal of Agricultural and Biological Sciences
Volume 12 No. 3, 2017, 123-129

DOI: https://doi.org/10.3844/ajabssp.2017.123.129

Submitted On: 7 September 2017 Published On: 6 November 2017

How to Cite: Mandang, J. P. (2017). Seedling Propagation of Kulo Chrysanthemum by Tissue Culture. American Journal of Agricultural and Biological Sciences, 12(3), 123-129. https://doi.org/10.3844/ajabssp.2017.123.129

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Keywords

  • Kulo Chrysanthemum
  • Multiplication
  • BAP
  • IAA
  • Coconut Water