Research Article Open Access

VARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY LENTINULA EDODES IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSE

Citlalli Harris-Valle1, Elisa Valenzuela-Soto2, Alfonso Sánchez3, Rigoberto Gaitán-Hernández4 and Martín Esqueda3
  • 1 Instituto Tecnológico Superior de Zacapoaxtla, Zacapoaxtla, Puebla, Mexico
  • 2 Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Hermosillo, Sonora, Mexico
  • 3 Coordinación de Tecnología de Alimentos de Origen Vegetal, Mexico
  • 4 Instituto de Ecología, A.C., Xalapa, Veracruz, Mexico

Abstract

The white-rot basidiomycete Lentinula edodes is the second most edible mushroom cultivated on the world. This fungus decomposes cell-wall associated macromolecules, is a natural degrader of lignin polymers. The differences in enzyme activities between strains of L. edodes provided useful information about the participation of enzymes in different development stages of the fungus. The effect of lignin on the fungal biomass production and activity behavior of ligninolytic enzymes when L. edodes is cultivated in a medium containing lignin with and without glucose as a carbohydrate source was tested. When glucose was present in the culture, lignin increase the mycelial biomass by 70% at 22 days compared to the control culture. The lignin media without glucose affected mycelial growth up to 20% less that the control without lignin and glucose. The activity of laccase, lignin peroxidase, aryl alcohol oxidase, manganese dependent peroxidase and catalase was modified depending on whether the medium had lignin and glucose, or lignin alone. A carbohydrate source is important to fungal growth, but the dissolution of lignin monomers might switch the signal that controls growth rate and enzymatic activity.

American Journal of Agricultural and Biological Sciences
Volume 9 No. 3, 2014, 369-378

DOI: https://doi.org/10.3844/ajabssp.2014.369.378

Submitted On: 3 January 2014 Published On: 28 April 2014

How to Cite: Harris-Valle, C., Valenzuela-Soto, E., Sánchez, A., Gaitán-Hernández, R. & Esqueda, M. (2014). VARIABILITY IN THE LIGNINOLYTIC ENZYMES ACTIVITY BY LENTINULA EDODES IN SUBMERGED CULTURE WITH LIGNIN AND GLUCOSE. American Journal of Agricultural and Biological Sciences, 9(3), 369-378. https://doi.org/10.3844/ajabssp.2014.369.378

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Keywords

  • Shiitake
  • Laccase
  • Lignin Peroxidase
  • Aryl Alcohol Oxidase
  • Manganese Dependent Peroxidase
  • Catalase