Research Article Open Access

A Cyanobacterium Synechocystis sp. PCC 6803 Glutaredoxin Gene (slr1562) Protects Escherichia coli against Abiotic Stresses

Ahmed Gaber1 and Salah El-Din El-Assal1
  • 1 Department of Genetics, Faculty of Agriculture, Cairo University, El-Gamaa Street P.O. Box 12613 Giza, Egypt

Abstract

Problem statement: Glutaredoxins (GRXs) are ubiquitous small heat stable glutathione-dependent oxidoreductase enzymes that play a crucial role in plant development and response to oxidative stress. Approach: Cyanobacterium Synechocystis strain PCC 6803 contains two genes (slr1562 and ssr2061) encoding glutaredoxins. In the present investigation the slr1562 gene (grxC) was isolated and characterized. Results: The results revealed that the amino acid sequence deduced from GrxC protein share high identity with those of GRXs from other organisms and contain the consensus GRX family domain with a CPFC active site. Northern blotting analysis revealed that the expression of slr1562 gene could be induced by oxidative and salt stresses. Moreover, the protein GrxC was successfully overexpressed as a soluble fraction in Escherichia coli JM109. The over-expression of GrxC in Escherichia coli cells significantly increased resistance of cells to oxidative, drought and salt stresses. Conclusion/Recommendations: These results suggest that the slr1562 gene could play an important role in regulating abiotic tolerance against oxidative, drought and salt stresses in different organisms.

American Journal of Agricultural and Biological Sciences
Volume 7 No. 1, 2012, 88-96

DOI: https://doi.org/10.3844/ajabssp.2012.88.96

Submitted On: 23 February 2012 Published On: 5 March 2012

How to Cite: Gaber, A. & El-Assal, S. E. (2012). A Cyanobacterium Synechocystis sp. PCC 6803 Glutaredoxin Gene (slr1562) Protects Escherichia coli against Abiotic Stresses. American Journal of Agricultural and Biological Sciences, 7(1), 88-96. https://doi.org/10.3844/ajabssp.2012.88.96

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Keywords

  • Luria-Bertani (LB)
  • different stress conditions
  • Glutaredoxins (GRXs)
  • Synechocystis PCC 6803
  • slr1562
  • recombinant enzyme
  • abiotic stress