Research Article Open Access

Enzymatic Bioconversion of Agave Leaves FiberHydrolysis Using Plackett-Burman Design

Miguel A. Medina-Morales1, J. C. Contreras-Esquivel1, H. De la Garza-Toledo2, R. Rodriguez1 and Cristobal N. Aguilar1
  • 1 Department of Food Science and Technology, School of Chemistry, Universidad Autonoma de Coahuila 25280, Saltillo, Coahuila, Mexico
  • 2 Department of Basic Sciences, Engineering Division, Universidad Autonoma Agrara “Antonio Narro”, 25350, Buenavista, Saltillo, Coahuila, Mexico


Problem statement: Biofuels production is becoming a key factor to help decrease pollution levels and the dependency of fossil fuels. Cellulose from lignocellulosic biomass is being used as a source of raw material for biofuels production, specifically bioethanol, so many ways to use it for this purpose are being developed. Approach: Cellulose content and enzymatic depolymerization of cellulose was evaluated in this contribution. Results: Cellulose content was of 67% on fibers, which places this material as a potential raw material for bioethanol production 42% of the cellulose content of the Agave leaves fibers was released as glucose due to enzymatic degradation. Seeing the behavior of the enzymatic hydrolysis at 96 h a mathematical model was applied which gave a time for enzymatic hydrolysis which must result in the maximum of glucose liberated under the conditions used for the process. Conclusion: Using Agave Atrovirens at 44 h of enzymatic hydrolysis will provide the highest yield of glucose which can be used for other processes such as ethanolic fermentation.

American Journal of Agricultural and Biological Sciences
Volume 6 No. 4, 2011, 480-485


Submitted On: 7 September 2011 Published On: 2 November 2011

How to Cite: Medina-Morales, M. A., Contreras-Esquivel, J. C., Garza-Toledo, H. D. L., Rodriguez, R. & Aguilar, C. N. (2011). Enzymatic Bioconversion of Agave Leaves FiberHydrolysis Using Plackett-Burman Design. American Journal of Agricultural and Biological Sciences, 6(4), 480-485.

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  • Plackett-Burman Design (PBD)
  • Enzymatic hydrolysis
  • cellulose degradation
  • Lignocellulosic residues
  • leaf cellulose fibers
  • fossil fuels
  • enzymatic means
  • cellulose depolymeryzation
  • cristallinity regions