Research Article Open Access

Antagonist capacity of Newly Isolated Strains of Pseudomonas Fluorescens against Three Important Phytopathogenic Bacteria

Reynaldo De La Cruz-Quiroz1, Catalina Chavez1, Marcela Hernandez1, Raul Rodriguez1, Daniel Hernandez1 and Cristobal Noe Aguilar1
  • 1 Universidad Autonoma de Coahuila, Mexico

Abstract

Problem Statement: Phytopatogenic bacteria cause several damages to plants with important economical consequences. They provoke losses of product quality affecting all commercial chain of crops, for this reason, their control is a priority. Approach: We evaluated antagonist capacity of newly isolated Pseudonomas fluorescens strains against three important phytopatogenic bacteria (Clavibacter michiganensis, Xanthomonas axonopodis and Erwinia carotovora). Soils from commercial cropping of Capsicum annum L of several Mexican regions were used to isolate P. fluorescens strains. Results: Isolates producing flourescein were purified on King B agar and biochemically identified. Crude extracts with and without cells were produced in King B broths and their antagonist capacities were evaluated by the plate diffusion procedure on nutritive agar. Conclusion: Obtained results demonstrated that cell free extracts exhibited a limited antagonist capacity in comparison of those extracts with cells, which showed an excellent capacity to inhibit the growth of C. michiganensis, X. axonopodis and E. carotovora, demonstrating the intracellular nature of the bioactive metabolites associated to bacterial growth inhibition.

American Journal of Agricultural and Biological Sciences
Volume 6 No. 2, 2011, 267-272

DOI: https://doi.org/10.3844/ajabssp.2011.267.272

Submitted On: 19 May 2011 Published On: 24 June 2011

How to Cite: Cruz-Quiroz, R. D. L., Chavez, C., Hernandez, M., Rodriguez, R., Hernandez, D. & Aguilar, C. N. (2011). Antagonist capacity of Newly Isolated Strains of Pseudomonas Fluorescens against Three Important Phytopathogenic Bacteria. American Journal of Agricultural and Biological Sciences, 6(2), 267-272. https://doi.org/10.3844/ajabssp.2011.267.272

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Keywords

  • Pseudonomas fluorescens
  • ellular nature
  • Mexican agriculture
  • Phytopatogen bacteria
  • foliar spots
  • Free Cell crude extracts (FC)
  • fluorescens strains
  • polypeptone
  • microorganisms
  • economical losses