Research Article Open Access

Vitamin B12 Status in Males and Females of different Age Groups

Ala Qatatsheh1
  • 1 Department of Nursing, Princess Aisha Bint Al-Hussein Faculty of Nursing, Al-Hussein bin Talal University, Ma'an, Jordan


Problem statement: Vitamin B deficiency remains widespread in many countries including Jordan, mainly due to inadequate dietary intake of vitamin B and absorption problems. Approach: Few researches on vitamin B12 status in healthy subjects are carried out. Thus, the aim of this study was to evaluate the serum levels of vitamin B12 in healthy Jordanian subjects living in the city of Ma'an. Randomized samples involving 511 healthy participants aged between 15-80 years whom referred for routine laboratory check up at the medical center of Al-Hussein Bin Talal University (AHU) in Jordan, were subjected to a health screening and then they provided a blood sample. Serum vitamin B12 concentration was determined using the fully automated Abbott AxSYM B12 assay, the accuracy and precision of this method was evaluated using certified reference materials. Results: Prevalence of low vitamin for 15-24-year-old, 25-34-year-old and over 34-year-old age groups among both males 2.7, 2.9 and 5.1% and females 5.1, 3.9 and 5.8% respectively were not significantly lower than those were not B12 deficient (P = 0.57 and P = 0.52, respectively). The highest prevalence was considered in both male and female over 34-year-old age group which increased by ageing. Conclusion/Recommendations: This study revealed that the prevalence of low vitamin B12 levels are considerably lower than other communities. One possible explanation of these observations is that Jordanians eat adequate animal products to increase the daily intake of vitamin B12. Further studies are needed to confirm these results.

American Journal of Agricultural and Biological Sciences
Volume 6 No. 2, 2011, 221-226


Submitted On: 17 February 2011 Published On: 6 June 2011

How to Cite: Qatatsheh, A. (2011). Vitamin B12 Status in Males and Females of different Age Groups. American Journal of Agricultural and Biological Sciences, 6(2), 221-226.

  • 2 Citations



  • Vitamin B12 deficiency
  • healthy jordanian
  • Microparticle Enzyme Intrinsic Factor Assay (MEIA)
  • fully automated Abbott
  • animal products
  • DNA synthesis
  • acidic environment
  • subjects suffering hematological