Research Article Open Access

DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle

Arash Javanmard1, Nader Asadzadeh2 and Fatollah Sarhadi2
  • 1 Department of Genomics, West and North-West Agriculture Biotechnology Research Institute, Tabriz, Iran
  • 2 Department of Biotechnology, Animal Science Research Institute, Karaj, Iran

Abstract

Problem statement: Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. Approach: The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 Animals from four breeds from two species of Iranian cattle populations in include Bos taurus (Sarabi, Golpayegani) and Bos indicus (Sistani, Taleshi) were genotyped for the Pit-1 HinfI and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). Results: The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in Bos indicus. Conclusion: Present result supported the previously proposed long evolutionary separation of these cattle subrace. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle.

American Journal of Agricultural and Biological Sciences
Volume 5 No. 3, 2010, 282-285

DOI: https://doi.org/10.3844/ajabssp.2010.282.285

Submitted On: 5 March 2010 Published On: 30 September 2010

How to Cite: Javanmard, A., Asadzadeh, N. & Sarhadi, F. (2010). DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian Bos indicus and Bos taurus Cattle. American Journal of Agricultural and Biological Sciences, 5(3), 282-285. https://doi.org/10.3844/ajabssp.2010.282.285

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Keywords

  • Pit-1
  • leptin
  • Bos taurus
  • Bos indicus
  • PCR-RFLP
  • polymorphism