Research Article Open Access

Analysis of Sarawak Bario Rice Diversity Using Microsatellite Markers

S. C. Wong1, P. H. Yiu1, S. T. W. Bong1, H. H. Lee1, P. N. P. Neoh1 and A. Rajan1
  • 1 University Putra Malaysia Bintulu Campus, Malaysia

Abstract

Problem statement: There are numerous claims on varieties of Bario rice cultivars in Sarawak. Many of them might be synonyms or homonyms. This preliminary study was to differentiate Bario Rice cultivars using microsatellite molecular markers. Approach: Microsatellite marker panels distributed on all 12 chromosomes on rice genome were employed to study 8 Bario rice cultivars in Sarawak. Results: Current study indicated that the microsatellite panel were able to differentiate the 8 Bario rice cultivars. A total of 31 alleles were generated at 12 polymorphic microsatellite loci among the cultivars using the selected polymorphic SSR primer pairs with an average of 2.6 alleles per locus. Average PIC value obtained was 0.5204. An UPGMA dendrogram based on SSR polymorphism indicated high variation among the rice varieties with the coefficient ranging from 0.16 and 0.92. Genetic diversity determination using cluster analysis showed differentiation of rice cultivars into 2 major groups and several sub-groups. Conclusion: The SSR markers were useful for differentiating the genotypes and assessment of genetic relationships due to its reproducibility and to multiallelic, codominant and informative properties.

American Journal of Agricultural and Biological Sciences
Volume 4 No. 4, 2009, 298-304

DOI: https://doi.org/10.3844/ajabssp.2009.298.304

Submitted On: 30 April 2009 Published On: 31 December 2009

How to Cite: Wong, S. C., Yiu, P. H., Bong, S. T. W., Lee, H. H., Neoh, P. N. P. & Rajan, A. (2009). Analysis of Sarawak Bario Rice Diversity Using Microsatellite Markers. American Journal of Agricultural and Biological Sciences, 4(4), 298-304. https://doi.org/10.3844/ajabssp.2009.298.304

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Keywords

  • Microsatellite markers
  • genetic diversity
  • Bario rice cultivars
  • Sarawak