Research Article Open Access

Mass Spectrometry/Mass Spectrometry Study on the Degradation of B-Aflatoxins in Maize with Aqueous Citric Acid

A. Méndez-Albores1, I. Nicolás-Vázquez2, R. Miranda-Ruvalcaba2 and E. Moreno-MartÍnez1
  • 1 UNAM-FESC, Campo 3. Av. Jorge Jiménez Cantú S/N, Cuautitlán Izcalli, C.P. 57740, Mexico
  • 2 UNAM-FESC, Campo 1. Av. 1º de Mayo S/N, Cuautitlán Izcalli, C.P. 54740, Mexico

Abstract

Degradation of B-aflatoxins in maize by means of 1N aqueous citric acid was confirmed by the AFLATEST immunoaffinity column method, High Performance Liquid Chromatography (HPLC), tandem mass spectrometry (MS/MS) and computational information. The AFLATEST and HPLC assays showed that 96.7% degradation occurred in maize contaminated with 93 ng g-1 when treated with the aqueous citric acid. Two major products, produced during the acidification process, were identified by their corresponding mass spectral data: a nonfluorescent compound lacking the lactone group evidenced by the presence of a peak m/z 286 and a nonfluorescent compound retaining the difurane moiety but lacking the lactone carbonyl and the cyclopentenone ring of the AFB1, also suggested by the peak m/z 206; the title fragments correspond to molecular ions in agreement with their respective molecular weights. According to the theoretical calculations, applying density functional theory, it was confirmed that the active site may be assigned to the carbonylic carbon of the lactonic moiety.

American Journal of Agricultural and Biological Sciences
Volume 3 No. 2, 2008, 482-489

DOI: https://doi.org/10.3844/ajabssp.2008.482.489

Submitted On: 6 June 2007 Published On: 30 June 2008

How to Cite: Méndez-Albores, A., Nicolás-Vázquez, I., Miranda-Ruvalcaba, R. & Moreno-MartÍnez, E. (2008). Mass Spectrometry/Mass Spectrometry Study on the Degradation of B-Aflatoxins in Maize with Aqueous Citric Acid. American Journal of Agricultural and Biological Sciences, 3(2), 482-489. https://doi.org/10.3844/ajabssp.2008.482.489

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Keywords

  • Detoxification
  • aflatoxin B1
  • aflatoxin B2
  • acidic treatment
  • tandem mass spectrometry
  • theoretical calculations