Research Article Open Access

Optimizing Nano-silver Formation by Fusarium oxysporum PTCC 5115 Employing Response Surface Methodology

M. Karbasian1, S. M. Atyabi2, S. D. Siadat3, S. B. Momen4 and D. Norouzian4
  • 1 Science and Research Campus, Islamic Azad University, Tehran, Iran
  • 2 Departments of Bacterial Vaccines and Antigens Production , Pilot Biotechnology and Research& Development , Pasteur Institute of Iran, Tehran, 13164, Iran
  • 3 Science and Research Campus, Young Researchers Club, Islamic Azad University, Tehran, Iran
  • 4 Departments of Bacterial Vaccines and Antigens production, Pilot Biotechnology Research and Development, Pasteur Institute of Iran, Tehran, 13164, Iran

Abstract

Fusarium oxysporum was grown in medium containing malt, yeast extracts and glucose on shaker at 25±1°C and180 rpm for 96 h. The mycelia were used to convert silver nitrate solution into nano-silver. The bioconversion was optimized through response surface methodology-central composite design .The factors which affected the process were pH, temperature, agitation rate, concentration of silver nitrate, time of reaction and weight of Fusarium oxysporum mass. The R2 was calculated to be 98% indicating the accuracy and ability of the polynomial model to be suitable and reasonable. Positive coefficient of the factors like concentration of silver nitrate solution (E) and weight of Fusarium oxysporum biomass (F), quadratic terms A2, B2, F2 and interaction term BC affected linearly formation of nano-silver whereas negative coefficient of pH(A), temperature (B), rate of agitation(C), time(D) along with quadratic terms C2, D2, E2, and interaction terms such as BF decreased the nano-silver formation.

American Journal of Agricultural and Biological Sciences
Volume 3 No. 1, 2008, 433-437

DOI: https://doi.org/10.3844/ajabssp.2008.433.437

Submitted On: 16 February 2007 Published On: 31 March 2008

How to Cite: Karbasian, M., Atyabi, S. M., Siadat, S. D., Momen, S. B. & Norouzian, D. (2008). Optimizing Nano-silver Formation by Fusarium oxysporum PTCC 5115 Employing Response Surface Methodology. American Journal of Agricultural and Biological Sciences, 3(1), 433-437. https://doi.org/10.3844/ajabssp.2008.433.437

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Keywords

  • Fusarium oxysporum
  • optimization
  • nano-silver formation
  • response surface methodology -central composite design