Hepatoprotective Impact Of TLR9 Antagonist ODN 2088 Against Carbon Tetrachloride (CCl4)-Induced Hepatic Injury
American Journal of Biochemistry and Biotechnology
The main objective of this study is to evaluate the hepatoprotective impact of TLR9 antagonist ODN 2088 against carbon tetrachloride (CCl4)-induced acute liver injury. In this study, 24 mice were grouped into four groups. CCl4 was introduced in all groups except for the control group intraperitoneally after pretreatment with ODN 2088 and silymarin for seven days. To assess the liver injury, serum alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) were estimated and histopathological changes in the liver tissue were investigated. Expression of pro-inflammatory cytokines including TNF-α and IL-6 were measured. It was observed that the serum levels of biochemical parameters such as ALT and AST were remarkably elevated in mice treated only with CCl4 compared with the control mice (treated with corn oil). This increase in levels of ALT and AST was remarkably diminished by pretreatment with ODN 2088. The hepatoprotective impact of ODN 2088 on CCl4-intoxicated mice was verified by histopathological studies. ODN2088 pretreatment inhibited liver inflammation by diminishing the liver expression of CCl4-induced activity of TNF-α and IL-6. In conclusion, TLR9 antagonist, ODN 2088, protected the mice against CCl4-induced liver damage and inflammation by down-regulating the expression of pro-inflammatory cytokines.
© 2020 Badr Alzahrani. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.