Current Research in Medicine

Formulation and Evaluation of a Novel Needle-Free Hepatitis B Surface Antigen Delivery System

C. D. Licy, K. Premalatha, Annie Shirwaikar and Arun Shirwaikar

DOI : 10.3844/amjsp.2010.88.96

Current Research in Medicine

Volume 1, Issue 2

Pages 88-96


Problem statement: Hepatitis B is a major public health problem, killing 1-2 million people annually, despite the introduction of an effective vaccine in 1982. Approximately 400 million persons worldwide have chronic hepatitis B. This is due to problems associated with vaccine delivery, stability and cost. Hence the present challenge in Vaccinology is to develop safer, cheaper and easy-to-deliver forms of vaccines. A novel needle-free oral vaccine will be an ideal tool to fight with this silent killer disease. Approach: Hence a study was conducted with the aim of formulating and evaluating an effective oral HBsAg vaccine. HBsAg-loaded microspheres were prepared by microencapsulation using albumin as polymer and sodium taurocholate as permeation enhancer. Bacitracin and aprotinin were incorporated as protease inhibitors. After in vitro studies, optimized drug-loaded microspheres were encapsulated in hard gelatin capsules which were further enteric coated with cellulose acetate phthalate. In vivo experiments were conducted in rabbits. Control group was administered with 20


© 2010 C. D. Licy, K. Premalatha, Annie Shirwaikar and Arun Shirwaikar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.