Taxifolin Potently Diminishes Levels of Reactive Oxygen Species in Living Cells Possibly by Scavenging Peroxyl Radicals
Satoru Sakuma, Yui Kishiwaki, Mayu Matsumura, Hiroyuki Sawada, Riko Hashimoto, Kohtaroh Gotoh, Kohhei Umemoto and Yohko Fujimoto
DOI : 10.3844/ajptsp.2018.1.6
American Journal of Pharmacology and Toxicology
Volume 13, 2018
Taxifolin, a naturally occurring dihydroflavonol, has been reported to possess antioxidant activity, whereas there is little information regarding its antioxidant potency as a flavonoid. In the present study, the antioxidant efficacy of taxifolin in living cells was compared to that of catechin, a potent radical scavenger, by using flow cytometry with the redox-sensitive fluorescent dye, 2′,7′-dichlorodihydrofluorescein diacetate. Further, diminishing efficacy of taxifolin on peroxyl radicals was assessed by using an electron spin resonance spin-trapping technique. Taxifolin significantly reduced tert-butyl hydroperoxide-induced increases in Reactive Oxygen Species (ROS) levels in Caco-2 cells; the effect of taxifolin was stronger than that of catechin. Taxifolin also scavenged peroxyl (ROO•) radicals with statistically significance compared to that of catechin. This study showed that taxifolin has the potential to diminish ROS levels in living cells, possibly by scavenging ROO•.
© 2018 Satoru Sakuma, Yui Kishiwaki, Mayu Matsumura, Hiroyuki Sawada, Riko Hashimoto, Kohtaroh Gotoh, Kohhei Umemoto and Yohko Fujimoto. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.