Research Article Open Access

Hyper-Ag-specific Ab Production in NC/Nga Mice is not associated with Deletion Polymorphism in the Promotor Region of the FcγRIIB Gene

Tohru Sakai1, Mariko Nakamoto1 and Emi Suto1
  • 1 University of Tokushima Graduate School, Japan

Abstract

Problem statement: NC/Nga (NC) mice produced high levels of ovalbumin (OVA)-specific IgG, IgG1 and IgG2a. We previously found deletion polymorphisms in the promoter region of fcgr2b in NC mice. To determine whether this mutation causes a hyper-humoral immune response, we generated congenic BALB/c mice carrying the NC-type fcgr2b allele (NC fcgr2b) and analyzed humoral immune response and FcγRIIB on germinal center (GC) B cells. Approach: BALB/c, NC and BALB/c- NC fcgr2b congenic mice were immunized with OVA 2 times at a 2-week-interval. Levels of OVA-specific IgG, IgG1 and IgG2a in serum were determined by ELISA. Four-color (anti-B220, anti-IgD, 2.4G2 and PNA) flow cytometry analysis was performed on splenocytes obtained from OVA-immunized mice and levels of FcγRIIB in GC (IgD-PNAhigh) and non-GC (IgD+PNAlow) B cells were analyzed. Results: Although perturbed up-regulation of FcγRIIB on GC B was observed in congenic mice, levels of OVA-specific Abs were comparable to those in BALB/c mice. Conclusion: NC fcgr2b affects the level of FcγRIIB in GC B cells but that the reduced FcγRIIB expression is not related to enhanced Ag-specific Ab responses in NC mice.

American Journal of Immunology
Volume 5 No. 3, 2009, 61-64

DOI: https://doi.org/10.3844/ajisp.2009.61.64

Submitted On: 27 May 2009 Published On: 31 December 2009

How to Cite: Sakai, T., Nakamoto, M. & Suto, E. (2009). Hyper-Ag-specific Ab Production in NC/Nga Mice is not associated with Deletion Polymorphism in the Promotor Region of the FcγRIIB Gene. American Journal of Immunology, 5(3), 61-64. https://doi.org/10.3844/ajisp.2009.61.64

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Keywords

  • NC/Nga mice
  • FcγRIIB
  • antibody
  • congenic mice