Research Article Open Access

Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

C. K. Chauhan1, M.J. Joshi1 and A. D.B. Vaidya2
  • 1 Saurashtra University, India
  • 2 ICMR Advanced Center for Reverse Pharmacology in Traditional Medicine, India

Abstract

Problem statement: The formation of a urinary stone, known as nephrolithiasis, urolithiasis, renal calculi or kidney stone is a serious, debilitating problem in all societies throughout the world. Struvite or Ammonium Magnesium Phosphate Hexahydrate (AMPH) is one of the components of urinary stone (calculi). Struvite stones are commonly found in women. Struvites form in humans as a result of urinary tract infection with ureolithic urea splitting micro organisms. These stones can grow rapidly forming "staghorn-calculi", which is more painful urological disorder. Therefore, it is of prime importance to study the growth and inhibition of Struvite crystals. Approach: This in vitro study had been carried out in the presence of herbal extract of Boerhaavia diffusa Linn. by using single diffusion gel growth technique. Sodium metasilicate solution of specific gravity 1.05 and an aqueous solution of ammonium dihydrogen phosphate of 0.5 M concentration were mixed so that the pH value 7.0 could be set. After the gelation, equal amount of supernatant solution of 1.0 M magnesium acetate prepared with 0.5 and 1% concentrations of the herbal extract of B. diffusa Linn. were gently poured on the set gels in the respective test tubes in the aseptic medium. Results: The growth of crystals without and with herbal extracts was monitored at regular time intervals. As the concentration of B. diffusa Linn. increased, the inhibition of crystals also increased in the gel media as well as the dissolution of crystals at the gel-liquid interface increases. The de-fragmentation of some grown crystals was also noticed. Conclusion: The herbal extract of B. diffusa Linn. inhibited the growth of struvite crystals in vitro. This study incorporated multidisciplinary interests and may be used for formulating the strategy for prevention or dissolution of urinary stones.

American Journal of Infectious Diseases
Volume 5 No. 3, 2009, 170-179

DOI: https://doi.org/10.3844/ajidsp.2009.170.179

Submitted On: 7 March 2009 Published On: 30 September 2009

How to Cite: Chauhan, C. K., Joshi, M. & Vaidya, A. D. (2009). Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn. American Journal of Infectious Diseases, 5(3), 170-179. https://doi.org/10.3844/ajidsp.2009.170.179

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Keywords

  • Ammonium Magnesium Phosphate Hexahydrate (AMPH)
  • urinary stone
  • gel growth