Extraction of Genomic DNA Using Magnetic Nanoparticles (Fe3O4) as a Solid-Phase Support
- 1 The M.S. University of Baroda, India
Magnetic separation technology, using magnetic particles, is quick and easy method for sensitive and reliable captures of specific proteins, genetic material and other biomolecules. The current paper describes a universal genomic DNA extraction method optimized in our laboratory using magnetic nanoparticles as a solid phase adsorbent. The yields of the isolated DNA with magnetic method were higher or equivalent to the conventional procedures in all the samples tested. Additionally, the magnetic method takes less than 15 minutes to extract DNA as against several hours taken by conventional protocols. Furthermore, the isolated DNA was found to function satisfactorily in PCR amplification and restriction endonuclease digestion. The developed procedure is simple, quick, cheap, robust and does not require the use of organic solvents or sophisticated equipments; thereby making it more amenable to automation.
Copyright: © 2007 Z. M. Saiyed and C. N. Ramchand. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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- magnetic nanoparticles
- mammalian cells
- agarose gel