American Journal of Environmental Sciences

Effect of Environmental Parameters on hydrogen Production using Clostridium Saccharoperbutylacetonicum N1-4(ATCC 13564)

Walid M. Alalayah, Mohd Sahaid Kalil, Abdul Amir H. Kadhum, Jamaliah Md. Jahim and Najeeb M. Alauj

DOI : 10.3844/ajessp.2009.80.86

American Journal of Environmental Sciences

Volume 5, Issue 1

Pages 80-86


Problem statement: Hydrogen gas production by Clostridium can be improved by several ways through media formulation, or suitable environment condition. This study was carried out to investigate the environmental factors effects on hydrogen production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Approach: The environmental factor studied includes initial substrate concentration, initial medium pH, temperature, sparging nitrogen and addition of Fe2+. Results: The result showed that the best yield of hydrogen produced (YP/S) was 3.10 moL (moL glucose)-1 when an initial glucose concentration was 10 g L-1, initial pH 6.0±0.2 at temperature 37°C. The volume of hydrogen produced was decreased when higher initial glucose concentration was applied. The yield of hydrogen increased when Fe2+ added to medium at concentration of 25 mg L−1. The yield and growth were further increased by sparging with nitrogen gas. Conclusion: It was observed that the best condition for highest hydrogen yield when initial pH 6.0±0.2 at 37°C and enhanced by adding ferrous sulfate in anaerobic process.


© 2009 Walid M. Alalayah, Mohd Sahaid Kalil, Abdul Amir H. Kadhum, Jamaliah Md. Jahim and Najeeb M. Alauj. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.