American Journal of Environmental Sciences

Toxicity of Reference Sediment Samples

K. R. Beg and S. Ali

DOI : 10.3844/ajessp.2008.347.352

American Journal of Environmental Sciences

Volume 4, Issue 4

Pages 347-352


This study was done to determine toxicity of certified reference sediment to be used for quality assurance in sediment toxicity testing program. Certified Reference Harbor Sediment (HS-4B for PAHs) and Estuarine Sediment (LGC-6137 for Trace Metals) were procured commercially from LGC Company, UK, and repeatedly analyzed against solid phase Microtox toxicity assay to determine EC50 values. Both PAHs rich and trace metals rich sediments were very toxic to Microtox with EC50 405 mg L-1 and 554 mg L-1 respectively. Solvent extraction resulted in a drastic decrease in the toxicity of PAHs rich reference sediment sample but in trace metal rich sediment the decrease in the toxicity was marginal. The certified reference sediments with the availability of toxicity data can be incorporated for quality assurance as controls in Microtox toxicity determination of field samples of unknown toxicity.


© 2008 K. R. Beg and S. Ali. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.