DEVELOPMENT OF A BACTERIAL CULTURE SYSTEM USING A PAPER PLATFORM TO ACCOMMODATE MEDIA AND AN INK-JET PRINTING TO DISPENSE BACTERIA
Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi and Toshiharu Enomae
DOI : 10.3844/ajbbsp.2014.81.87
American Journal of Biochemistry and Biotechnology
Volume 10, Issue 1
Generally, bacterial culture is performed manually and is subject to error. Here, we created a novel, well-ordered and reliable system for dispensing bacteria microscopically by using paper and an ink-jet printer for controlled patterning. For paper to accommodate a culture medium, hydrophobic/hydrophilic patterns were incorporated onto the paper by immersing paper in a toluene solution of polystyrene and drying for complete hydrophobization, followed by etching discrete, small areas of hydrophilicity by ink-jet printing with toluene. Agar was hydrolyzed with sulfuric acid for appropriate viscosity and dispensed with an ink-jet printer. In a separate experiment, bacterial cells were sequentially printed on a medium and colonies were observed microscopically. The results of this experiment ensured the successful dispensing of bacteria using ink-jet printing. An almost constant number of particles per droplet were ejected using a polystyrene latex as a model of bacterial dispersion. Consequently, we expect this technology to be adapted for the development of a paper-based bioassay system.
© 2014 Tithimanan Srimongkon, Takuya Ishida, Kiyohiko Igarashi and Toshiharu Enomae. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.