Synchrotron Powder Diffraction Study of Radiation Damage in Langmuir Blodgett Nanotemplate Crystallised Protein
Jonathan P. Wright, Eugenia Pechkova and Claudio Nicolini
DOI : 10.3844/ajbbsp.2014.162.168
American Journal of Biochemistry and Biotechnology
Volume 10, Issue 3
Polycrystalline samples of lysozyme were prepared with and without a Langmuir-Blodgett (LB) thin film template via both the hanging drop method and batch crystallisation. Powder diffraction methods are used to compare these samples and to measure their resistance to radiation damage at room temperature. The X-ray induced amorphisation of the samples was followed as a function of time and it was shown that diffraction does not entirely disappear even at very long exposure times. Two distinct kinetic timescales are evident suggesting that early and late stage processes are quite different. Radiation damage was also shown to be localized in the sample in the region where the beam impinges.
© 2014 Jonathan P. Wright, Eugenia Pechkova and Claudio Nicolini. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.