Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells
A. Bódalo, J. Bastida, M.F. Máximo, A. M. Hidalgo and M. D. Murcia
DOI : 10.3844/ajbbsp.2005.1.4
American Journal of Biochemistry and Biotechnology
Volume 1, Issue 1
Lyophilized non-proliferating microbial cells of Pseudomonas sp. 42A2 showed their ability to catalyze the bioconversion of oleic acid into (E)-10-hydroxy-8-octadecenoic acid (MHOD) and (E)- 7,10-dihydroxy-8-octadecenoic acid (DHOD). The reaction conditions were optimized in order to produce the maximum amount of MHOD. At 30°C and using phosphate buffer (0.1 M and pH=8), an initial substrate concentration of 10 mg mL-1 and a cell concentration of 8 mg mL-1, practically all the oleic acid in the medium was biotransformed within 42 h, with hardly any DHOD produced (1.4 mg mL-1).
© 2005 A. Bódalo, J. Bastida, M.F. Máximo, A. M. Hidalgo and M. D. Murcia. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.