Enzymatic Hydrolysis of Pretreated Kenaf using a Recombinant Xylanase: Effects of Reaction Conditions for Optimum Hemicellulose Hydrolysis
Nur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail, Siti Fatimah Zaharah Mohamad Fuzi, Roshanida A. Rahman, Nazlee Faisal Ghazali and Rosli Md Illias
DOI : 10.3844/ajabssp.2016.54.66
American Journal of Agricultural and Biological Sciences
Volume 11, Issue 2
An efficient hemicellulose hydrolysis has now become a crucial step for xylooligosaccharides production for food additives and nutraceuticals industries. In this study, xylanase hydrolysis of pretreated hemicellulosic kenaf (Hibiscus cannabinus) for substantial hemicellulose hydrolysis was demonstrated. Experiments were performed primarily to investigate the effect of several critical reaction conditions towards the enzymatic hydrolysis of the pre-treated kenaf. The effects of various ranges of reaction conditions (substrate loading, xylanase loading, pH, temperature and time) were systematically and thoroughly studied. The study of hemicellulosic kenaf conversion using single enzyme was proven to effectively yield up to 59% of conversion after 48 h incubation using 3% (w/v) of substrate loading and 400 U of xylanase loading at 50°C in pH 4.0 buffer systems. The use of a single xylanase on the pre-treated kenaf was a key parameter in understanding the action of the main hemicellulose degrading enzyme towards hydrolysing the hemicellulose backbone of the pre-treated kenaf. Enzyme feeding strategy was also conducted and the results revealed that a batch feeding strategy of 400 U xylanase loading yielded the highest hemicellulose hydrolysis compared to the other fed batch enzyme feeding strategy. It was concluded that each reaction conditions and enzyme feeding strategy gave a significant impact towards the enzymatic hydrolysis and the use of optimum reaction conditions resulted in a high product yield at the end of the reaction. With the discovery of the importance of each reaction conditions involved in a hydrolysis, this study highlights the need for a comprehensive investigation on the reaction conditions using a mixture of enzymes towards achieving a complete hydrolysis with a higher product yield.
© 2016 Nur Izyan Wan Azelee, Jamaliah Md Jahim, Ahmad Fauzi Ismail, Siti Fatimah Zaharah Mohamad Fuzi, Roshanida A. Rahman, Nazlee Faisal Ghazali and Rosli Md Illias. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.