SSR Analysis of Maternal and Paternal Lines Selected in the Don Region (Russia)
N. V. Markin, A. V. Usatov, V. N. Vasilenko, A. I. Klimenko, O. F. Gorbachenko, D. N. Maidanyuk and L. V. Getmantseva
DOI : 10.3844/ajabssp.2016.13.18
American Journal of Agricultural and Biological Sciences
Volume 11, Issue 1
Evaluation of DNA polymorphisms of breeding material of sunflower from L.A. Zhdanov Don Experimental Station of oil Crops of V.S. Pustovoit All-Russian Research Institute of Oil Crops represented by 17 maternal (CMS) lines and 12 paternal (Rf) lines was conducted. There were identified 35 allelic variants of CMS lines and 42 allelic variants of Rf lines with use of 11 SSR markers. It is shown that the level of genetic diversity of microsatellite loci of CMS lines is 1.2 times lower than that of Rf lines. The average number of alleles per SSR locus for maternal lines also lowers than for paternal lines. In addition, CMS lines and Rf lines differ in the frequency of alleles of SSR loci. Thus, the discriminatory capacity of the studied marker system for identification of cultivated sunflower lines was quite high and is suitable for certification of the lines.
© 2016 N. V. Markin, A. V. Usatov, V. N. Vasilenko, A. I. Klimenko, O. F. Gorbachenko, D. N. Maidanyuk and L. V. Getmantseva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.