American Journal of Agricultural and Biological Sciences

Effect of Temperature and Storage Time on Shelf Life of Mycoherbicidal Products of Colletotrichum dematium FGCC#20

Jaya Singh and A.K. Pandey

DOI : 10.3844/ajabssp.2010.315.320

American Journal of Agricultural and Biological Sciences

Volume 5, Issue 3

Pages 315-320


Problem statement: The storage environment can have a drastic impact on the stabilization of microbial biopesticides. As a general rule, storing microbial preparation at lower temperature increase long term survivelity. Approach: Optimization of shelf life is critically important for biocontrol products containing living microorganisms. Conidia of Colletotrichum dematium, (FGCC#20) a fungal pathogen of the weed, Parthenium hysterophorus were produced in solid agrowaste substrate. After solid based formulation, formulated mycoherbicidal products were conditioned at room temperature and at 4°C temp in different storage periods. Results: The longest shelf-life (conidial inoculums viability) was found in formulated products stored at 4°C temperature. At this temperature 4°C pesta (Wheat bran + Keolin) granules were 80% viable for 3 months and 10-15% viable after one month. Conclusion: The temperature had a renounced effect on the shelf life of the formulated products during storage. Maximum shelf life was observed in pesta granules for at least 1 year at 4°C would be adequate for commercialization.


© 2010 Jaya Singh and A.K. Pandey. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.