American Journal of Agricultural and Biological Sciences

Effect of Lactate Treatments on Survival of Food-Borne Pathogens in Frozen Shrimp (Penaeus merguiensis)

Alireza Shirazinejad and Noryati Ismail

DOI : 10.3844/ajabssp.2010.242.246

American Journal of Agricultural and Biological Sciences

Volume 5, Issue 2

Pages 242-246


Problem statement: Sodium lactate treatments were evaluated for reducing population of pathogenic bacteria such as Salmonella enteritidis, E. coli O157:H7, Vibrio parahaemolyticus and Vibrio cholerae, inoculated on frozen shrimp (Penaeus merguiensis). Approach: Fresh shrimp were inoculated with the above pathogens, separately, at level of 105 CFU g-1 and appliedin sodium lactate solutions (NaL: 0.5% and 2.5% v/v) and 1% Lactic Acid (LA) plus 1.5% sodium lactate (as buffered lactic acid) followed by freezing. The samples were stored at -18°C and antimicrobial and organoleptical effects of the treatments were evaluated after 90 days. Results: Results indicated that there was synergistic effect between frozen storage combined with 2.5% (v/v) buffered LA which resulted in a great significant reductions (p≤0.05) approximately 5.11, 5.39, 2.86 and 2.66 log CFU g-1 in the number of colonies of V. cholerae, V. parahaemolyticus, S. enteritidis and E. coli O157:H7, respectively. On the other hand, although the effectiveness of sodium lactate increase with increased concentrations, however, there are sensory problems associated with increasing concentrations of sodium lactate. Conclusion/Recommendations: Therefore, 1.5% (v/v) sodium lactate plus 1.0% (v/v) Lactic Acid (buffered LA) was recommended to be used for frozen shrimps without adversely interfering with sensory quality.


© 2010 Alireza Shirazinejad and Noryati Ismail. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.