Research Article Open Access

Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)

Lesley Maurice Bilung1, Son Radu1, Abdul Rani Bahaman1, Raha Abdul Rahim1, Suhaimi Napis1, Cheah Yoke Kqueen1, Chandrika Murugaiah1, Yousr Abdul Hadi1, Tunung Robin1 and Mitsuaki Nishibuchi1
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American Journal of Immunology
Volume 1 No. 1, 2005, 31-36

DOI: https://doi.org/10.3844/ajisp.2005.31.36

Submitted On: 1 May 2006 Published On: 31 March 2005

How to Cite: Bilung, L. M., Radu, S., Bahaman, A. R., Rahim, R. A., Napis, S., Kqueen, C. Y., Murugaiah, C., Hadi, Y. A., Robin, T. & Nishibuchi, M. (2005). Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa). American Journal of Immunology, 1(1), 31-36. https://doi.org/10.3844/ajisp.2005.31.36

Abstract

Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.

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Keywords

  • RAPD-PCR
  • cockles
  • Vibrio parahaemolyticus