@article {10.3844/ojbsci.2015.53.58,
article_type = {journal},
title = {Cytogenetic Analysis among Train Depot Workers Exposed to Total Volatile Organic Compounds},
author = {Awang, Normah and Isah, Siti Adillah and Hamid, Asmah},
volume = {15},
number = {2},
year = {2015},
month = {Jun},
pages = {53-58},
doi = {10.3844/ojbsci.2015.53.58},
url = {https://thescipub.com/abstract/ojbsci.2015.53.58},
abstract = {Occupational exposure to toxic chemicals such as Total Volatile Organic Compounds (TVOC) from diesel engine exhaust implicates genotoxic risk. The objective of this study was to determine the DNA damage among KL Sentral depot workers exposed to TVOC by means of cytogenetic analysis. Data were obtained partially from disseminated questionnaires and buccal cells samples were collected from a total of 27 depot workers and 10 office workers by using wooden tongue depressor. TVOC measurement was carried out by using Photoionisation Detector (PID). 4 sampling points were selected, namely Locomotive (LOCO), Power Generating Car (PGC), refuelling of diesel and coach. Cytogenetic analysis was done by using Acridine Orange (AO) staining. Micronucleus (MN) frequency as a biomarker for DNA damage was scored from 1,000 cells per sample observed under fluorescence microscope. The results showed that the overall means of concentrations for TVOC were 0.1±0.1, 0.0±0.0, 0.1±0.0 and 0.1±0.0 in LOCO, PGC, refuelling of diesel and coach, respectively. TVOC concentration measured did not exceed the permissible level based on DOSH 2010. For the cytogenetic analysis, depot workers showed significantly higher MN (p},
journal = {OnLine Journal of Biological Sciences},
publisher = {Science Publications}
}