TY  - JOUR
AU  - Sarkar, Aditya Kumar 
AU  - Roy, Susmita 
AU  - Pal, Ananya 
AU  - Pakrashi, Sourav 
AU  - Mahra, Prahlad Kumar 
AU  - Sahoo, Sayantee 
AU  - Deb, Aritra 
AU  - Mishra, Madhusmita 
AU  - Sen, Sudip Kumar 
AU  - Thakur, Ashoke Ranjan 
AU  - Chaudhuri, Shaon Ray 
PY  - 2008
TI  - Microbial Biodiversity Screening for Metal Accumulators from Mineral Ore Rich Site in Andhra Pradesh, India
JF  - OnLine Journal of Biological Sciences
VL  - 8
IS  - 2
DO  - 10.3844/ojbsci.2008.32.40
UR  - https://thescipub.com/abstract/ojbsci.2008.32.40
AB  - Problem statement: Since the site selected for screening was 
a source of magnetite, the objective of this study was to isolate some groups 
of magnetotactic bacteria and microbes with potential of metal accumulation. Approach: 
The culture based approach was used for the study. Pure isolates obtained were 
characterized on the basis of morphology, biochemical tests, physiology as well 
as at the 16S rDNA based molecular level. They were screened for their metal accumulating 
ability. Results: Five pure bacterial isolates were obtained 
by culture dependent method, isolation being done on a modified Magnetospirillum 
magnetotacticum specific medium (DSMZ-380). These isolates not only exhibited 
morphological variation as found from Environmnetal Scanning Electron Microscopy 
(ESEM), but their cell surface also varied greatly in composition which was confirmed 
from various staining. Similarly another observed fact was that inspite of the 
same source of isolation, these strains exhibited a varied temperature and pH 
tolerance which indicates the diversity in the population. 16S rDNA based analysis 
shows the molecular identification and real time PCR based pathogenecity analysis 
proved that they lacked S. aureus or P. aeruginosa like pathogenic gene(s). The 
isolates were screened for metal accumulating property and all were found to tolerate 
a wide range of heavy metals and among them certain strains bear high potential 
of accumulation of metals like copper and chromium. Conclusion: 
This study reports the isolation and characterization of 5 novel bacterial isolates 
from the mining area of Andhra Pradesh, India revealing its rich biodiversity. 
It also points towards the ability of media DSMZ-380 to support growth of metal 
accumulating non-magnetotactic isolates.