TY  - JOUR
AU  - ElMenshawy, Nadia E. 
AU  - ElAziz, Sherin A. Abd 
AU  - El Deghadi, Heba A. 
AU  - Mahfoz, Rabab M. 
AU  - Metwali, Abd El-Hameed A. 
PY  - 2011
TI  - Zeta Chain-Associated Protein-70 Expression is a Prognostic Tool in Mature and Precursor B-Cell Neoplasm
JF  - Current Research in Medicine
VL  - 2
IS  - 1
DO  - 10.3844/amjsp.2011.40.46
UR  - https://thescipub.com/abstract/amjsp.2011.40.46
AB  - Problem statement: The Zeta Chain-Associated Protein (ZAP) is a 70-kDa molecule
associated with the zeta chain of the CD3 receptor complex of the lymphocyte population. It plays an
important role in signaling initiation, activation T-cell receptor, phosphorylation of multiple
downstream targets. So that believed as T. lymphocyte specific .Several studies is gathering that the
pattern of expression is not lineage specific. We aimed to study the role of ZAP-70 /CD38 as new
prognostic marker in CLL and its prognostic value in ALL patients. Approach: Our study included 49
ALL ranged from 2-42 years they were 27 males and 22 females. One hundred fifty six patients with
CLL. They were 87 males and 69 females, their ages ranged from 50-77 yearsImmunophenotyping. by
flow cytometry (EPICS XL-2and 4 color Bechman Coulter USA), intracellular ZAP70 protein was
assessed by flow cytometry using (Caltag laboratories, Beckman Coulter USA). Results:
Immunophenotyping of 49 ALL patients ZAP-70 was found at significantly higher levels in T-ALL
cases compared with B-lineage (p<0.001), however, a proportion of B- lineage ALL showed high
levels of ZAP-70. Moreover we observed that ZAP-70 is higher in pre-B ALL than pro B ALL and
common ALL with more decline in mature B-ALL. Follow up patient by zap, pretreatment versus post
treatment p = 0.008, correlation of zap with hematological and clinical parameter in ALL, no significant
correlation except positively correlated with leucocytic count while in CLL highly significant correlation
with splenomegally but not with lymphadenopathy, strong association between zap-70 and binnet staging
but not with CD38. Overall survival of zap-70 in ALL/CLL show that short free survival associated with
positive zap-70. ZAP-70 has raised great interest in CLL patients because it represents a powerful
prognostic marker. Conclusion: Our results provide preliminary indication on the potential use of this
protein as a prognostic marker also in ALL. These results clearly show that ZAP-70 may indeed be a
therapeutic target. ZAP-70 expression in a subset of patients with ALL opens the perspective of
investigating the use of an inhibitor and can be a candidate molecule for targeted therapy.