@article {10.3844/ajidsp.2009.170.179,
article_type = {journal},
title = {Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn},
author = {Chauhan, C. K. and Joshi, M.J. and Vaidya, A. D.B.},
volume = {5},
number = {3},
year = {2009},
month = {Sep},
pages = {170-179},
doi = {10.3844/ajidsp.2009.170.179},
url = {https://thescipub.com/abstract/ajidsp.2009.170.179},
abstract = {Problem statement: The formation of a urinary stone, known as 
nephrolithiasis, urolithiasis, renal calculi or kidney stone is a serious, debilitating 
problem in all societies throughout the world. Struvite or Ammonium Magnesium 
Phosphate Hexahydrate (AMPH) is one of the components of urinary stone (calculi). 
Struvite stones are commonly found in women. Struvites form in humans as a result 
of urinary tract infection with ureolithic urea splitting micro organisms. These 
stones can grow rapidly forming "staghorn-calculi", which is more painful 
urological disorder. Therefore, it is of prime importance to study the growth 
and inhibition of Struvite crystals. Approach: This in vitro 
study had been carried out in the presence of herbal extract of Boerhaavia diffusa 
Linn. by using single diffusion gel growth technique. Sodium metasilicate solution 
of specific gravity 1.05 and an aqueous solution of ammonium dihydrogen phosphate 
of 0.5 M concentration were mixed so that the pH value 7.0 could be set. After 
the gelation, equal amount of supernatant solution of 1.0 M magnesium acetate 
prepared with 0.5 and 1% concentrations of the herbal extract of B. diffusa Linn. 
were gently poured on the set gels in the respective test tubes in the aseptic 
medium. Results: The growth of crystals without and with herbal 
extracts was monitored at regular time intervals. As the concentration of B. diffusa 
Linn. increased, the inhibition of crystals also increased in the gel media as 
well as the dissolution of crystals at the gel-liquid interface increases. The 
de-fragmentation of some grown crystals was also noticed. Conclusion: 
The herbal extract of B. diffusa Linn. inhibited the growth of struvite 
crystals in vitro. This study incorporated multidisciplinary interests and may 
be used for formulating the strategy for prevention or dissolution of urinary 
stones.},
journal = {American Journal of Infectious Diseases},
publisher = {Science Publications}
}