TY  - JOUR
AU  - Ingianni, Angela 
AU  - Quartuccio, Marta 
AU  - Madeddu, Maria Antonietta 
AU  - Sanna, Adriana 
AU  - Dessì, Sandro 
AU  - Pompei, Raffaello 
PY  - 2007
TI  - Isolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular Method
JF  - American Journal of Infectious Diseases
VL  - 3
IS  - 3
DO  - 10.3844/ajidsp.2007.159.164
UR  - https://thescipub.com/abstract/ajidsp.2007.159.164
AB  - The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 g of food sample. A total of 278 meat samples were tested in comparison with PCR and conventional cultural assays. A total of 42 (15.4%) L. monocytogenes were detected. PCR analysis gave 3 false negative results and culture failed to detect the Listeria in 5 cases. With this cultural-molecular method the identification and quantitative detection of L. monocytogenes were achieved within 36 hours and no false positive or negative tests were obtained, thus fitting most food industry requirements.