@article {10.3844/ajidsp.2007.159.164,
article_type = {journal},
title = {Isolation and Identification of Listeria monocytogenes in Processed Meat by a Combined Cultural-molecular Method},
author = {Ingianni, Angela and Quartuccio, Marta and Madeddu, Maria Antonietta and Sanna, Adriana and Dessì, Sandro and Pompei, Raffaello},
volume = {3},
number = {3},
year = {2007},
month = {Sep},
pages = {159-164},
doi = {10.3844/ajidsp.2007.159.164},
url = {https://thescipub.com/abstract/ajidsp.2007.159.164},
abstract = {The isolation and identification of Listeria monocytogenes in processed meat samples by a combined cultural-molecular method is described. It allows the identification of Listeria strains by means of a hybridization technique with a specific DNA probe directed to the listerial internalin gene. The specificity of this method was found to be 100% and sensitivity was as low as 1 CFU/2.5 g of food sample. A total of 278 meat samples were tested in comparison with PCR and conventional cultural assays. A total of 42 (15.4%) L. monocytogenes were detected. PCR analysis gave 3 false negative results and culture failed to detect the Listeria in 5 cases. With this cultural-molecular method the identification and quantitative detection of L. monocytogenes were achieved within 36 hours and no false positive or negative tests were obtained, thus fitting most food industry requirements.},
journal = {American Journal of Infectious Diseases},
publisher = {Science Publications}
}