TY - JOUR AU - Alshiyab, Hisham Salem AU - Kalil, Mohd Sahaid AU - Hamid, Aidil Abdul AU - Yusoff, Wan Mohtar Wan PY - 2009 TI - Improvement of Biohydrogen Production under Increased the Reactor Size by C. acetobutylicum NCIMB 13357 JF - American Journal of Environmental Sciences VL - 5 IS - 1 DO - 10.3844/ajessp.2009.33.40 UR - https://thescipub.com/abstract/ajessp.2009.33.40 AB - Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the partial pressure of produced gas. A novel solution to enhance the bacterial productivity was through reduction of gas pressure. Approach: Increasing the reactor size showed to enhance the bacterial production of hydrogen. Results: The technique of increasing reactor size resulted to enhance the hydrogen yield (YP/S) from 269 mL g-1 glucose utilized to maximum yield of 448 mL g-1 glucose utilized by using 125 mL and 2 L reactor size respectively. The hydrogen productivity was also enhanced from 71 mL-1 h-1 to maximum of 91 mL L-1 h-1 was obtained by using 125 mL and 1 L reactor size respectively. Biomass concentration was enhanced from 1.03 g L-1 to maximum of 1.68 g L-1 by using 125 mL and 2 L reactor size were used respectively, hydrogen yield per biomass (YP/X) of 267 mL g-1 L-1, biomass per substrate utilized (YX/S) of 0.336 and produced hydrogen in gram per gram of glucose utilized (YH2/s) of 0.04 when 2 L reactor size was employed. Conclusion: By using bigger reactor size, the effect of gaseous products in fermentation medium was reduced and enhanced both bacterial productivity and biomass concentration.